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Laboratory work for GET-IN doctoral network

Doctoral Candidate 7

Bioprocess systems engineering approaches to improve rAAV production for gene therapy applications 


Host institution of GET-IN the University of Thessaloniki

Host institution: Aristotle University of Thessaloniki (AUTh), Greece

Supervisory team: Prof. Alexandros Kiparissides (PhD promoter, AUTh), Dr. Jan Schrooten (Antleron), Dr. Sarah Fadda (Siemens Process Systems Engineering)

Bioprocess systems engineering approaches to improve rAAV production for gene therapy applications 

This project is focussed on improving upstream and downstream bioprocessing yields during the production of recombinant Adeno-associated viruses (rAAV). The objectives of the project are: (1) To develop a modular, ‘plug-and-play’ mathematical model of all upstream and downstream bioprocessing steps required for the production of rAAVs. (2) To use Process Systems Engineering methodologies to identify optimal routes to manufacture rAAVs that maximise productivity and reduce manufacturing costs. (3) To develop model-based tools for the assessment of uncertainty and risk that can predict the ‘real-life’ performance of rAAV manufacturing technologies, including ones developed through other GET-IN DC projects.

A successful project will result in: (1) A digital mapping of both established and novel rAAV manufacturing technologies; (2) A model-based tool to identify bioprocessing conditions that maximise rAAV productivity; (3) A set of digital decisional tools to guide the selection of optimal up- and down-stream technologies for the robust and cost-efficient production of rAAVs.

Enrolment in Doctoral School: Aristotle University of Thessaloniki (AUTH), Greece

Planned secondments:

  • Siemens Process Systems Engineering, London UK (Months 9-11): Bioprocess modelling

  • KU Leuven, Belgium (Months 18-19): Introduction to upstream bioprocessing

  • KU Leuven, Belgium (Months 29-30): Introduction to downstream bioprocessing


  • You hold a master’s degree in physics, (bio) engineering or a related field.

  • You are ambitious, well organized and have excellent communication skills.

  • You are proficient in English both spoken and written

  • You have the ability to work independently and have a critical mindset.

  • You are an enthusiastic and motivated person, eager to participate in network wide training events, international travel and public awareness activities.

  • Willingness to travel


  • Solid knowledge of mathematical modelling, process modelling, process flowsheets

  • An understanding of bioprocessing, biomanufacturing

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