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Laboratory work for GET-IN doctoral network

Project DC8

Digital twin and multi-parametric optimisation of upstream unit operations for rAAV manufacturing

PROJECT INFORMATION

Host institution of GET-IN the University of Thessaloniki

Host institution: Aristotle University of Thessaloniki (AUTh), Greece, and Antleron, Belgium

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Country: Greece and Belgium

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Supervisory team: Prof. Alexandros Kiparissides (PhD promoter, AUTh), Dr. Jan Schrooten (Antleron), Dr. Sarah Fadda (Siemens Process Systems Engineering)

PROJECT DESCRIPTION 

With more than 350 potential therapies in clinical trials in the first half of 2020, the ability to reliably and sustainably manufacture large quantities of rAAVs is increasingly becoming a bottleneck in the commercialisation of gene therapy products. However, AAV manufacturing still heavily relies on transient transfection production methods using primarily adherent cell lines, both of which are processes heavily reliant on heuristic or statistics-driven optimisation methods. The aim of this project is to develop a detailed digital twin of upstream unit operations for AAV-based gene therapy manufacturing in order to enable model-based optimisation of the manufacturing process.

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The developed digital twin will comprise both kinetic/mechanistic models, where sufficient knowledge of the underlying mechanisms exists as well as data driven (and hybrid) models where knowledge is currently lacking.Rather than reporting just titre or productivity metrics the model output will provide quantitative descriptors for key Critical Quality Attributes (CQAs) developed in collaboration with WP1 and WP2. The digital twin will be calibrated and validated against experimental data from literature, data produced in house at Antleron and data from WP1 and WP3. State-of-the-art parametric uncertainty analysis techniques will be used to investigate the correlations between uncertainty/variability in Critical Process Parameters (CPPs)  and their effect on product CQAs. Finally, multi-parametric optimisation techniques will be applied in order to define the optimal ‘process operating window’ while taking into account variability both in the efficacy of preceding unit operations and well as in the employed manufacturing technologies.

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A successful project will result in a validated and calibrated digital twin model for upstream unit operations of rAAV production.


Enrolment in Doctoral School: AUTh


Planned secondments:

  • Siemens Process Systems Engineering, UK (Months 9-11): Whole process modelling, model development and parameter estimation;

  • KU Leuven, Belgium (Month 23): Wet lab expertise for rAAV production.

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This project has received funding from the European Union’s Framework Programme for Research and Innovation, Horizon Europe under Grant Agreement No. 101119880

ON5b, Herestraat 49, Box 1023

3000 Leuven, Belgium

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